To study the minimum inhibitory concentration of Colistin on MDR klebsiella pneumoniae by agar dilution and broth dilution method

Abstract

Background - The threat to human health posed by the global spread of
multidrug-resistant (MDR) gram-negative bacteria is growing. In the healthcare
environment, K. pneumoniae is also the most prevalent pathogen that is
resistant to many drugs including carbapenems . In the past few decades,
polymyxins have been the most widely utilised antibiotic choice for treating K.
pneumoniae that is resistant to carbapenem. In fact, polymyxin E, also known
as colistin, is frequently the only antibiotic to achieve sufficient serum levels
and minimum inhibitory concentrations (MIC), making it a "last resort"
treatment for MDR K. pneumoniae infections. Therefore , this study was
conducted to check the MIC of colistin towards K .pneumonaie Materials – In
tertiary Care Hospital , samples that were received at Microbiology lab were
processed using standard microbiological procedures and further studied . The
MIC of agar dilution and Broth dilution was compared . Result – From the 250
MDR K.pneumonaie processed , Suspectibility of MDR K.pneumoniae to
Colistin By Agar Dilution Method was 73.2 % . And Suspectibility of MDR
K.pneumoniae to Colistin By Broth Dilution Method was 78.4 %. When Both
of them were compared Broth Dilution was more susceptible to colistin than
Agar diltion method.